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| Purification step | Total activity (U) | Total protein (mg) | Specific activity (U mg-1) | Yield (%) | Purification (fold) | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| CotA | YjqC | CotA | YjqC | CotA | YjqC | CotA | YjqC | CotA | YjqC | |
| Crude extract | 30.4 | 46240 | 121 | 121 | 0.25 | 382.1 | 100 | 100 | 1 | 1 |
| 40–80% (NH4)2 SO4 | 23.3 | 25110 | 48.6 | 36.4 | 0.48 | 689.8 | 76.6 | 54.3 | 1.9 | 1.8 |
| DEAE sepharose FF | 17.0 | 7985 | 13.5 | 8.9 | 1.26 | 897.2 | 55.9 | 17.3 | 5.0 | 2.3 |
| Superdex 200 | 9.6 | 5691 | 5.3 | 1.8 | 1.81 | 3161.7 | 31.6 | 12.3 | 7.2 | 8.3 |
