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. 2016 Jun 30;11(6):e0158166. doi: 10.1371/journal.pone.0158166

Fig 7. Mitochondrial ROS measurement.

Fig 7

Mean fluorescence intensity (MFI—arbitrary units) by pancreatic islets from fed (CT) or 48-hour fasted (48h) rats. The islets were treated with MitoSOX Red reagent and incubated for 60 minutes in the presence of 2.8 or 16.7 mM glucose with or without addition of VAS2870 (20 μM) or DPI (5 μM) associated or not with leucine (LEU—20 mM)—experimental protocol 4. The results are presented as mean ± SEM of four cell preparations for each group as indicated by one-way ANOVA and Dunnett's post test.