. 2016 Jun 30;11(6):e0157366. doi: 10.1371/journal.pone.0157366

Table 3. Fish species detected using eDNA metabarcoding (six filtrations) and complete electrofishing during the sampling campaigns operated together in 2014 at the R1 site.

Fish taxa		eDNA metabarcoding approach						electrofishing sampling
Latin name	Common name			Number of reads				Detections
Latin name	Common name	R1a	R1b	R1c	R1d	R1e	R1f	Detections
*SHARED TAXA (n = 10)*
*Rutilus rutilus*	Roach	30883	72062	38571	52227	22037	95338	X
*Salmo trutta*	Trout	26227	22944	29873	56015	15028	57358	X
Barbatula sp.	-	2569	3882	5235	4813	1801	9748	X
*Teletes souffia*	Riffle dace	3780^*	3468^*	2512^*	8532^*	1909^*	4501^*	X
*Squalius cephalus*	Chub	823	705	814	2155	535	1634	X
*Phoxinus phoxinus*	Minnow	42	60	134	83	63	286	X
*Perca fluviatilis*	Perch	52	84	177	176	29	20	X
*Lepomis gibbosus*	Pumpkinseed	56	54	77	230	13	47	X
*Alburnoides bipunctatus*	Spirlin				276			X
Gobio spp.	-			14	53		64	X
*TAXA DETECTED USING eDNA ONLY (n = 6)*
*Barbus barbus*	Barbel		61		455
*Scardinius erythrophthalmus*	Rudd						33
Cottus spp.	-				19
*Alburnus alburnus*	Bleak					17
*Oncorhynchus mykiss*	Rainbow trout	14
Leuciscus spp.	-			12
Total richness				16				10
Fish taxa		eDNA metabarcoding approach						electrofishing sampling
Latin name	Common name			Number of reads				Detections
Latin name	Common name	R1a	R1b	R1c	R1d	R1e	R1f	Detections
*SHARED TAXA (n = 10)*
*Rutilus rutilus*	Roach	30883	72062	38571	52227	22037	95338	X
*Salmo trutta*	Trout	26227	22944	29873	56015	15028	57358	X
Barbatula sp.	-	2569	3882	5235	4813	1801	9748	X
*Teletes souffia*	Riffle dace	3780^*	3468^*	2512^*	8532^*	1909^*	4501^*	X
*Squalius cephalus*	Chub	823	705	814	2155	535	1634	X
*Phoxinus phoxinus*	Minnow	42	60	134	83	63	286	X
*Perca fluviatilis*	Perch	52	84	177	176	29	20	X
*Lepomis gibbosus*	Pumpkinseed	56	54	77	230	13	47	X
*Alburnoides bipunctatus*	Spirlin				276			X
Gobio spp.	-			14	53		64	X
*TAXA DETECTED USING eDNA ONLY (n = 6)*
*Barbus barbus*	Barbel		61		455
*Scardinius erythrophthalmus*	Rudd						33
Cottus spp.	-				19
*Alburnus alburnus*	Bleak					17
*Oncorhynchus mykiss*	Rainbow trout	14
Leuciscus spp.	-			12
Total richness				16				10

Number of reads per species and per filtration.

*The molecular marker does not discriminate between Telestes souffia, Chondrostoma nasus and Chondrostoma toxostoma.