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. 2016 Jun 30;11(6):e0157366. doi: 10.1371/journal.pone.0157366

Table 5. Fish species detected using eDNA metabarcoding (three filtrations in 2014) and the seven gillnets sampling campaigns (from 1995 to 2014) within the Aiguebelette lake.

Fish taxa eDNA metabarcoding Gillnets sampling
Latin name Common name Detections Reads (%) Detections Frequency
SHARED TAXA (n = 17)
Perca fluviatilis Perch X 37,2821 X F
Coregonus lavaretus Lavaret X 18,6306 X F
Rutilus rutilus Roach X 14,1278 X F
Esox lucius Pike X 7,7440 X F
Scardinius erythrophthalmus Rudd X 6,8929 X F
Squalius cephalus Chub X 4,4577 X F
Leuciscus spp. - X 3,3451 X F
Abramis brama Bream X 1,5857 X F
Alburnus alburnus Bleak X 1,3127 X C
Tinca tinca Tench X 0,9018 X F
Salaria fluviatilis Freshwater blenny X 0,8853 X R
Lepomis gibbosus Pumpkinseed X 0,7120 X F
Cyprinus carpio Carp X 0,6686 X F
Salvelinus spp. - X 0,6596 X F
Gobio spp. - X 0,5522 X F
Salmo trutta Trout X 0,1040 X R
Barbatula sp. - X 0,0012 X R
TAXA DETECTED USING eDNA ONLY (n = 4)
Micropterus sp. - X 0,0743
Carassius spp. - X 0,0570
Cottus spp. - X 0,0041
Barbus barbus Barbel X 0,0013
SPECIES CAUGHT BY TRADITIONAL SAMPLING ONLY (n = 5)
Blicca bjoerkna Silver bream X C
Lota lota Burbot X C
Oncorhynchus mykiss Rainbow trout X R
Sander lucioperca Pikeperch X F
Chondrostoma nasus Nase X R
Total richness 21 22

Relative abundance of reads (in % for a total of 895,133 reads) per species (eDNA metabarcoding) and frequency of species caught with gillnets: frequent species (F), common species (C) and rare species (R) caught in more than 50%, 15–50% and less than 15%, respectively, of the total number of fishing campaigns.