Table 6. Fish species detected using eDNA metabarcoding (12 filtrations in 2014) and the 13 electrofishing sampling campaigns (from 1988 to 2015) within the Tier River.

Fish taxa		eDNA metabarcoding		electrofishing sampling
Latin name	Common name	Detections	Reads (%)	Detections	Frequency
SHARED TAXA (n = 19)
Rutilus rutilus	Roach	X	39,8441	X	C
Salmo trutta	Trout	X	28,0080	X	F
Telestes souffia	Riffle dace	X	10.4617 *	X	F
Alburnoides bipunctatus	Spirlin	X	7,0129	X	C
Barbus barbus	Barbel	X	5,4274	X	F
Barbatula sp.	-	X	4,3937	X	F
Squalius cephalus	Chub	X	2,8675	X	F
Cottus spp.	-	X	0,4949	X	C
Gobio spp.	-	X	0,4658	X	F
Oncorhynchus mykiss	Rainbow trout	X	0,3078	X	R
Lampetra spp.	-	X	0,2530	X	C
Phoxinus phoxinus	Minnow	X	0,1923	X	F
Lepomis gibbosus	Pumpkinseed	X	0,1506	X	R
Perca fluviatilis	Perch	X	0,0713	X	R
Leuciscus spp.	-	X	0,0340	X	R
Scardinius erythrophthalmus	Rudd	X	0,0061	X	R
Alburnus alburnus	Bleak	X	0,0038	X	R
Abramis brama	Bream	X	0,0037	X	R
Salaria fluviatilis	Freshwater blenny	X	0,0014	X	R
SPECIES CAUGHT BY TRADITIONAL SAMPLING ONLY (n = 2)
Esox lucius	Pike			X	R
Thymallus thymallus	European grayling			X	R
Total richness		19		21

Relative abundance of reads (in % of a total of 788,244 reads) per species (eDNA metabarcoding) and frequency of species caught with gillnets: frequent species (F), common species (C) and rare species (R) caught in more than 50%, 15–50% and less than 15%, respectively, of the total number of fishing campaigns.

*The molecular marker does not discriminate between Telestes souffia, Chondrostoma nasus and Chondrostoma toxostoma.