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. 2016 Jun 30;11(6):e0158481. doi: 10.1371/journal.pone.0158481

Fig 3. Expression of osteoblast differentiation markers following BST2 knockdown.

Fig 3

hAD-BMSCs transfected with siRNA were cultured for 5 days in the presence or absence of OS, and qRT-PCR was performed to measure the expression of ALP, COL1α1, BSP, OCN, and OSX, with GAPDH as the internal control. Relative expression levels were normalized to the expression of the untreated negative control sample. Compared with the untreated control, increased expression levels of ALP, OSX, COL1α1, BSP, and OCN were apparent in cells treated with OS and the control siRNA; however, the expression of these markers was decreased after BST2 knockdown by siRNA. Values are expressed as means ± SEM. *, P<0.05; **, P<0.01, when compared with cells treated with OS and the control siRNA.