Figure 2. TGF-β2-mediated cytoskeleton remodelling and the expression of α-SMA are reduced in αB-crystallin depleted cells.
FHL124 cells were seeded on chamber slides, and treated with αB-crystallin siRNA and TGF-β2 as in Figure 1. Cytoskeleton remodelling (F-actin) was detected using Texas Red conjugated phalloidin antibody (A1–4) at 20× and 40× magnification and α-SMA was detected using a monoclonal antibody against α-SMA and Texas Red-goat anti-mouse IgG (B1–4) at 20× and 40× magnification; scale bar=10 μm.