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. Author manuscript; available in PMC: 2016 Jun 30.
Published in final edited form as: Biochem J. 2016 Mar 17;473(10):1455–1469. doi: 10.1042/BCJ20160128

Figure 2. TGF-β2-mediated cytoskeleton remodelling and the expression of α-SMA are reduced in αB-crystallin depleted cells.

Figure 2

FHL124 cells were seeded on chamber slides, and treated with αB-crystallin siRNA and TGF-β2 as in Figure 1. Cytoskeleton remodelling (F-actin) was detected using Texas Red conjugated phalloidin antibody (A14) at 20× and 40× magnification and α-SMA was detected using a monoclonal antibody against α-SMA and Texas Red-goat anti-mouse IgG (B14) at 20× and 40× magnification; scale bar=10 μm.