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. 2016 Jun 30;11(6):e0158386. doi: 10.1371/journal.pone.0158386

Fig 2. HIV+HCV co-culture increased mRNA and protein expression levels of α-SMA, collagen IV, TGF-β, and EPM in HSCs.

Fig 2

(A) QRT-PCR analysis of fibrogenesis-related genes in LX-2 cells. MRNA expression levels of α-SMA, collagen IV, TGF-β, and EPM were significantly upregulated after culture with HIV+HCV. GAPDH was used as the internal control. *P < 0.05 compared with the HIV or HCV group. (B) Western blotting analysis of the fibrogenesis-related protein expression levels in LX-2 cells. EPM, α-SMA, collagen IV, and TGF-β levels were upregulated after 72 h of co-culture with HIV+HCV. GAPDH was used as an internal control. (C) Western blotting analysis of EPM protein expression levels in LX-2 cells incubated with HIV+HCV at the indicated time points (h). GAPDH was used as an internal control. Bar graphs are shown on the right. * P < 0.05 compared with 24h.