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. 2016 Jun 30;11(6):e0158386. doi: 10.1371/journal.pone.0158386

Fig 3. HIV+HCV co-culture promoted HSC proliferation and invasion in an EPM-dependent manner in vitro.

Fig 3

(A) Western blotting analysis of EPM protein expression in LX-2 cells with EPM knockdown (Lv-shEPM) and EPM overexpression (Lv-EPM). The empty vector-transfected LX-2 cells were used as controls. (B) Cell proliferation analyses were performed using a CCK-8 kit in LX-2 cells with EPM knockdown (Lv-shEPM) and EPM overexpression (Lv-EPM). The empty vector-transfected LX-2 cells were used as controls. *P < 0.05 compared with the HIV or HCV group. (C) In vitro cell invasion assay of LX-2 cells with EPM knockdown after HIV+HCV co-culture. The empty vector-transfected LX-2 cells were used as controls. **P < 0.01 compared with Lv-scramble. (D) In vitro cell invasion assay of LX-2 cells with EPM overexpression after HIV+HCV co-culture. The empty vector-transfected LX-2 cells were used as controls. **P < 0.01 compared with Lv-GFP. Experiments were performed three times.