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. 2016 May 6;2(5):e1501372. doi: 10.1126/sciadv.1501372

Fig. 4. DAF-12/DIN-1 up-regulates the expression of tbh-1 during starvation.

Fig. 4

(A) tbh-1 promoters with three AGTACA hexamer elements (−4139, −3193, and −2711 upstream of ATG) for DAF-12. Mutagenesis or deletion of these elements led to a significant reduction in Ptbh-1::gfp expression during food-deprived conditions. (B to D) A mutation in daf-12(rh61rh411) or din-1(dh127) inhibited the expression of Ptbh-1::gfp expression (B) and reduced the mRNA levels of tbh-1 (C) and the levels of octopamine (D) in starved worms. The right part shows quantification of GFP levels (B). Results are means ± SD of three experiments. aP < 0.01 versus well-fed WT worms; bP < 0.05 versus starved WT worms. (E) The three putative DAF-12 binding sites in the promoter regions of tbh-1 were detected by ChIP with anti-GFP antibody. ChIP results were quantitated by qPCR. *P < 0.05 versus well-fed worms.