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. 2016 Jun 30;11(6):e0158436. doi: 10.1371/journal.pone.0158436

Fig 2. hRPA and hRAD52 failed to stimulate DNA strand exchange by hRAD51.

Fig 2

(A) Regions of hRAD52 that correspond to yRad52N, M and C, and their similarities (%) to yRad52. (B and C) Same amount (2 μg) of purified hRAD52 and its derivatives (B), and yRPA and hRPA (C) were separated by SDS-PAGE and stained with coomassie brilliant blue. (D) DNA strand exchange assay using yRad52 and hRAD51 was performed as shown in Fig 1D, except that yRPA was replaced with hRPA. (E) Products of D were quantified (hRPA) and compared with the data shown in Fig 1F (yRPA). Error bars are standard deviations (n = 3). F. Same analyses as in E except that hRAD52 was used as a mediator.