Fig 4. Effects of C-terminal deletion of yRad52 and hRAD52 on their ssDNA binding activities.

(A and C) ³²P-labeled 70-mer ssDNA (100 nM) was incubated without (lane 1 and 7) or increasing concentrations (0.25, 0.5, 1, 2 and 3 μM from left to right) of yRad52 (lane 2–6 of A), yRad52NM (lane 8–12 of A), hRAD52 (lane 2–6 of C), or hRAD52NM (lane 8–12 of C). Protein-ssDNA complexes were analyzed by electrophoresis through 6% polyacrylamide gel. (B and D) The same experiment in A and C were repeated and percentage of the ssDNA-protein complex was calculated from their band intensities. Error bases are STD (n = 3).