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. 2016 Jun 30;11(6):e0157231. doi: 10.1371/journal.pone.0157231

Fig 5. ASC-dependent inflammasome recognized cytosolic rickettsiae.

Fig 5

BMMs of WT and ASC-/- mice were isolated, cultivated, and infected with R. australis at an MOI of 10 or treated with LPS plus ATP as described in Materials and Methods. At 24 h p.i., the secretion of IL-1β (A), IL-18 (B) and IL-10 (C) was assayed by ELISA. D, Activation of caspase-1 was determined by immunoblotting detection of the active unit p10 in the processed supernatant of infected WT and ASC-/- BMMs at 24 h p.i.. Data represent two independent experiments with consistent results. Each experiment included at least 4 replicates. *, p<0.05.