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. 2016 Jun 30;11(6):e0157231. doi: 10.1371/journal.pone.0157231

Fig 6. NLRP3 was involved in recognition of rickettsiae by inflammasome at the early stage of infection in BMMs.

Fig 6

WT and NLRP3-/- BMMs were isolated, cultivated, and infected with R. australis at MOIs of 6 and 2. The transcriptional levels of NLRP3 in WT BMMs at different time intervals of infection (at MOI of 6) were determined by RT-PCR as described in Materials and Methods (A). At 12 h (B) p.i., the secretion levels of IL-1β by WT and NLRP3-/- BMMs were determined by ELISA. The cleavage of caspase-1 was determined by detection of the active unit p10 in the processed supernatant at 12 h p.i. (C). Data represent mean ± SD for at least 3 replicates each group. *, p<0.05 for a significant difference between WT and NLRP3-/- mice; ns, not significantly different.