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. 2016 Jun 30;11(6):e0158424. doi: 10.1371/journal.pone.0158424

Fig 6. Impact of the absence of Crz1 on mRNA levels and Ena1 protein accumulation after high pH stress.

Fig 6

A) Time-course of the accumulation of Ena1 mRNA after shifting cells to pH 8.0. Total RNA from wild type (closed circles) and crz1 (open circles) cells was extracted and mRNA levels were determined by qRT-PCR and quantified as indicated in Materials and Methods. B) The strains described above were transformed with plasmid pKC201, carrying the lacZ reporter fused to the ENA1 promoter. Cells were exposed to pH 8.0 for the indicated times, collected, and β-galactosidase activity measured. C) Strain MLM001 (CRZ1) and MLM002 (crz1), carrying in both cases C-terminally GFP-tagged version of ENA1 were shifted to pH 8.0 for the indicated times and the amount of total Ena1 quantified by immunoblot using anti-GFP antibodies and internal standards of recombinant GFP protein. Data is presented as the mean ± SEM from 3 (panel A) or 4 (panels B and C) independent experiments.