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. 2016 Jun 30;11(6):e0158502. doi: 10.1371/journal.pone.0158502

Fig 1. General concept of turning a 3D Printer into a molecular device.

Fig 1

We explored the possibility of retrofitting low-cost 3D printers to perform rapid, automated nucleic acid isolation (< 15 min) and amplification (< 25 min). (A) The potential applications of the modified 3D printer in the context of low-cost molecular diagnosis of infectious diseases. Step 1 includes processing varied specimens through magnetic particle based nucleic acid extraction and purification. In step 2, the eluted DNA or RNA can be used in downstream analysis by various molecular detection approaches. Examples are: a) real-time thermal cycling using a commercial thermal cycler (this work), b) probe-based amplification operated by the 3D printer with post-PCR imaging (this work)., c) other molecular detection methods such as a lateral flow assay after PCR or isothermal amplification (underway). (B) Schematic showing the advantages of using the 3D printer for automated extraction. It is faster and requires minimal user-intervention once the process begins. It reduces performance variations induced by users. (C-E) Photos that show the major components that were added to the 3D printer to enable our current work. (C) The Printrbot Play 3D printer (base footprint: 5” × 11”) is the most compact 3D printer we have successfully converted into a nucleic acid extraction device. An 8-sample MPPA (magnetic particle processor attachment) was attached to the luer-lock syringe to carry out magnetic particle based nucleic acid extraction. The magnets were stored inside disposable 0.1-mL PCR tubes to eliminate direct contact between the magnets and samples. (D) The Printrbot Simple 3D printer (base footprint of 11” × 13”) is small enough to fit inside a biosafety cabinet. The 8-sample and 12-sample (shown) MPPA function properly when attached to either the Printrbot Play or Printrbot Simple 3D printer. (E) An adaptor for holding the PCR capillary tubes is attached to the Printrbot Simple 3D printer. The PCR capillary tubes were shuttled by the 3D printer between the 95°C denaturation and the 60°C annealing/extension baths (not shown).