Fig. 4. The effect of G₁₄ mutagenicity is correlated with DNA replication timing.

(A and B) The regression of mutation rate and replication timing for G₁₄-ORF alleles inserted at six different sites on chromosome XII and two sites on chromosome XV (Spearman’s R = 0.89, P = 0.007) (A) and for insertion of G₀-URA3 at the same sites (Spearman’s R = 0.63, P = 0.086) (B). Replication timing is shown in minutes after the release of cells into synchronized S phase, as reported by Nieduszynski et al. (30). Error bars show 95% confidence intervals.