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. 2016 Apr 29;2(4):e1501860. doi: 10.1126/sciadv.1501860

Fig. 2. tRNAGlyACC can be aminoacylated by GlyRS but not deaminated by ADAT.

Fig. 2

(A) A34- and I34-containing tRNAGly are efficiently aminoacylated by human GlyRS. tRNAGlyICC + tRNAGlyACC (dashed line) are the tRNAs remaining from the partial deamination of tRNAGlyACC by ADAT. The proportion of tRNAGlyICC and tRNAGlyACC was quantified: 42.5% of tRNAGlyICC and 57.5% of tRNAGlyACC. (B) tRNAAlaAGC is completely deaminated by ADAT after 15 min of incubation. tRNAGlyACC is only partially modified after 60 min of incubation. (C) Confirmation by sequencing of A to I deamination. Deaminated tRNAAlaAGC displays a G34 instead of an A34, indicating full I34 modification. Partial A to I deamination can be observed for tRNAGlyACC. A tRNAAla with a GlyACC anticodon loop displays an A34, indicating no I34 deamination. (D) Comparison of human anticodon loop sequences for each ADAT substrate and tRNAGly.