. Author manuscript; available in PMC: 2016 Sep 16.

Published in final edited form as: Pediatr Res. 2016 Mar 16;80(1):101–109. doi: 10.1038/pr.2016.50

TABLE 3.

PRIMERS FOR REAL TIME qRT-PCR.

Gene	Direction	Sequence (5’-3’)	Product	UniSTS
TNF-α	S	CACTAAGAATTCAAACTGGGGC	166 bp	28864
	AS	GAGGAAGGCCTAAGGTCCAC
IL-10	S	ACCTGGGTTGCCAAGCCTTGTC	158 bp	Ref 39 ^a
	AS	AAATCGATGACAGCGCCGTAGC
TLR1	S	CACATCAAGTGAAAAATATTCCTCC	151 bp	24274
	AS	TAAATGGTGAACTGCGACCC
TLR2	S	CTACTGGGTGGAGAACCTTATGGT	76 bp	1092
	AS	CCGCTTATGAAGACACAACTTGA
TLR3	S	TCCTAGAAGAGATGTAATTG	169 bp	66831
	AS	CCCAAAAACTCTGTACATTA
TLR4	S	GTTTCTGAGCAGTCGTGCAG	172 bp	480158
	AS	CAGGGCTTTTCTGAGTCGTC
GAPDH	S	AACAGCGACACCCACTCCTC	258 bp	270428
	AS	GGAGGGGAGATTCAGTGTGGT

, not listed at UniSTS

AS, antisense; bp, base pair; GADPH, glyceraldehyde phosphate dehydrogenase; IL-10, interleukin-10; qRT-PCR, quantitative reverse-transcription polymerase-chain reaction; S, sense; TLR, Toll-like receptor; TNF, tumor necrosis factor