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. Author manuscript; available in PMC: 2017 Jun 30.
Published in final edited form as: J Neurochem. 2016 Jan 17;136(6):1246–1258. doi: 10.1111/jnc.13518

Figure 2.

Figure 2

Figure 2

Figure 2

Components of the γ-secretase complex also play a role in regulating APP degradation by proteasome and lysosome. (a) Effects of γ-secretase, proteasome, and lysosome inhibitors on the accumulation of unprocessed endogenous CTFα. The first lane is the vehicle-treated control. The last lane is the sample prepared from wt-7 cells treated with γ-secretase inhibitor compound E (compE) used as standards of CTFβ-myc and CTFα-myc. (b) In lanes 3–10, cells were transfected with human APPsw expression plasmid. In lane 2, cells were transfected with unrelated protein LacZ. In lane 1, cells were mock transfected with an empty vector. Lane 11 is the sample prepared from wt-7 cells treated with compound E used as standards of CTFβ-myc and CTFα-myc. All APP CTFs were detected using C15. (c) Quantitative analysis of the formation and turnover of APP-CTFs. Results are expressed as the mean (± SD) of three independent Western blot results shown in Fig. 2B.