Figure 5.
Recovery of PS1C does not necessarily restore the γ-secretase activity toward APP in NCT−/− cells. Knockout cells were cultured in the presence or absence of MG132. Up panel, immunoblot probed with anti-PS1C that recognizes both regular and caspase produced PS1C; bottom panel, immunoblot probed with C15. Lane 12 is the sample prepared from wt-7 cells treated with compound E used as standards of CTFβ-myc and CTFα-myc.