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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: J Immunol. 2016 May 20;197(1):288–295. doi: 10.4049/jimmunol.1501946

FIGURE 2.

FIGURE 2

Transgenic GP Ib-IX function is impaired due to a GEMs-association dysfunction. A. The aggregometry analyses of vWf binding to transgenic platelets (2×105/μl) were performed in the presence of 1.5mg/ml ristocetin. B. The murine whole blood was perfused over a human vWf-coated surface in a parallel-plate flow chamber at shear rates of either 1,500s−1 or 15,000s−1. C. The rhodamine-conjugated phalloidin staining showed that transgenic platelets changed their morphology from filopodia protrusion to lamellipodia upon binding to immobilized vWf. Each experiment was performed at least 3 times and the error bars in rolling experiments were calculated from the mean rolling velocities of at least 100 cells in 5 different view fields.