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. 2016 Jun 16;16(1):263–277. doi: 10.1016/j.celrep.2016.05.064

Figure 1.

Figure 1

Stable Melanoma Marker Expression, Chromosomal Aberrations, and Gene Expression upon In Vivo Passaging of PDXs

(A) Tumor fragments derived from biopsies or surgical excisions were transplanted subcutaneously into NSG mice. After first passage (.X1), PDX fragments were passaged into a next set of mice (.X2).

(B) Speed of tumor outgrowth during passaging, shown for two different PDXs. Colors represent single PDX (.X1) passaged into a next set of three mice (.X2).

(C) H&E stainings and IHC stainings for MelanA, S100, gp-100, and tyrosinase were performed on the parental tumor and two subsequent passages of PDXs (X1 and X2). Scale bars indicate 100 μm.

(D) Copy-number profiles, based on array CGH, show the parental tumor (P) M013 and two PDX passages (the X2 passage was established from the third X1 PDX).

(E) Copy-number profiles, based on array CGH, show the parental tumor (P) M003 and two PDX passages (the X2 passages are next to their own X1-passaged PDX). Blue, deletion; red, amplification.

(F) Hierarchical clustering of RNA-sequencing data performed on patient samples and PDXs derived of these samples, after filtering as described in the Supplemental Experimental Procedures, is shown.