Figure 3.

RPA Decorates the Strands Persisting between Anaphase taz1Δ Chromatin Masses

(A) Representative images of cells with endogenously tagged Rad11^RPA, viewed as in Figure 1. In WT cells, Rad11^RPA foci are detectable as foci only during S phase.

(B) taz1Δ cells viewed as in (A). In anaphase, Rad11^RPA stretches from one chromatin mass to the other.

(C) Structural illumination microscopy (SIM) images of taz1Δ cells with Rad11^RPA tagged as in (A) and histone H3 as in Figure 1.

(D) Magnified and rotated image of the left pole of the cell shown in (C). The forked structure suggests the simultaneous presence of two entanglements.

(E) Film frames of anaphase with tags as in (C). Rad11^RPA retracts progressively.

See also Figure S1 and Movie S4.