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Retention times and mass spectrometric details of budesonide, 16α-hydroxyprednisolone and betamethasone (internal standard).
| Analyte | Retention time (min) | Cone voltage (V) | Collision energy (eV) | Quantification transition (m/z) | Confirmation transition (m/z) |
|---|---|---|---|---|---|
| BUD | 8.35 | 15 | 11 | 431 > 413 | 431 > 323 |
| 16α-HP | 6.41 | 14 | 10 | 377 > 359 | 377 > 323 |
| BET | 7.42 | 16 | 8 | 393 > 373 | 393 > 147 |
