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. 2016 Mar 17;20(7):1381–1391. doi: 10.1111/jcmm.12828

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© 2016 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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The protective effects of AICAR on cardiomyocyte apoptosis induced by tBHP. (A) The derease of cell viability were abrogated in tBHP group pre‐treated with AICAR. (B and C) The cell apoptotic rate was increased significantly in tBHP group, however, decreased in cardiomyocytes pre‐treated with AICAR. (D) Cell apoptosis was determined with Hoechst 33258 staining. 200× magnification. (E) Effects of AICAR on the expression of apoptosis‐related proteins in tBHP‐induced cardiomyocytes. Mean ± S.D., n = 3, *P < 0.05, **P < 0.01 versus control, #P < 0.05, ##P < 0.01 versus tBHP.