Table 2.
Comparison of physiological properties of PV+BCs and AACs
| P (Mann– | |||
|---|---|---|---|
| Parameters | PV+BC | AAC | Whitney) |
| Input resistance (Mohm) | 42.12 (33.22, 51.80) | 79.47 (70.18, 100.47) | 0.1 × 10−3 *** |
| Membrane time constant (ms) | 7.20 (5.75, 10.20) | 11.0 (9.05, 11,75) | 0.04* |
| Relative amplitude of hyperpolarizing sag | 1.28 (1.16, 1.41) | 1.21 (1.14, 1,30) | NS |
| Relative amplitude of rebound response following hyperpolarization | 1.35 (1.28, 1.57) | 1.22 (1.18, 1.37) | NS |
| Relative amplitude of early overshoot for depolarizing steps | 1.13 (1.08, 1.27) | 1.17 (1.11, 1.27) | NS |
| Rheobase current (pA) | 250 (200, 400) | 100.0 (85.0, 150.0) | 0.6 × 10−3 *** |
| Maximal action potential number for any input current | 109.0 (80.75, 136.50) | 162.0 (154.4, 169.5) | 0.001** |
| Voltage threshold (mV) | −41.78 (−43.43, −39.17) | −41.61 (−44.64, −40.05) | NS |
| Action potential amplitude (mV) | 51.32 (45.48, 67.24) | 67.19 (62.30, 71.63) | NS |
| Action potential rise time (ms) | 0.0005 (0.0004, 0.0005) | 0.0005 (0.0004, 0.0005) | NS |
| Action potential half‐width (ms) | 0.26 (0.25, 0.33) | 0.37 (0.34, 0.40) | 0.004** |
| Action potential width (ms) | 1.25 (1.12, 1.31) | 1.38 (1.31, 1.45) | 0.007** |
| Voltage difference between start and end of action potential (mV) | 14.75 (13.27, 16.78) | 11.57 (9.72, 13.03) | 0.001** |
| Maximal steepness of rising phase of action potential (mV ms−1) | 220.70 (179.32, 300.35) | 250.0 (187.87, 293.46) | NS |
| Voltage for maximal (positive) steepness of action potentials (mV) | −7.55 (−17.94, −2.40) | −13.44 (−19.56, −1.80) | NS |
| Maximal (negative) steepness of falling phase of APs (mV ms−1) | −233.4 (−242.2, −186.5) | −206.5 (−230.1, −175.6) | NS |
| Voltage for maximal negative steepness of APs (mV) | −9.43 (−22.09, −3.04) | 1.65 (−13.60, 7.41) | NS |
| Amplitude of after‐hyperpolarization (mV) (old method) | −0.11 (−0.61, 0.03) | 0.02 (−0.26, 1.09) | NS |
| Duration of after‐hyperpolarization (ms) (old method) | 0.22 (0.21, 0.28) | 0.39 (0.32, 7.13) | 0.01* |
| Amplitude of after‐hyperpolarization (mV) (new method) | 14.93 (12.63, 16.74) | 11.96 (9.73, 13.51) | 0.02* |
| Duration of after‐hyperpolarization (ms) (new method) | 1.39 (1.31, 1.57) | 1.80 (1.62, 8.53) | 0.008** |
| Latency of first action potential (ms) | 39.20 (24.90, 172.15) | 162.60 (32.15, 432.25) | NS |
| Action potential number in ‘steady’ trace* | 24 (14.25, 39.00) | 25.0 (15.75, 30.25) | NS |
| Average inter‐spike interval (ms) in ‘steady’ trace* | 33.37 (20.12, 54.30) | 31.83 (25.77, 46.0) | NS |
| Action potential half‐width (ms) in ‘steady’ trace* | 0.31 (0.26, 0.33) | 0.36 (0.35, 0.39) | 0.001** |
| Action potential amplitude (mV) in ‘steady’ trace* | 53.81 (45.23, 64.03) | 66.09 (64.11, 70.67) | 0.02* |
| Voltage threshold (mV) in ‘steady’ trace* | −41.04 (−42.65, −38.86) | −42.60 (−42.89, −39.46) | NS |
| Difference in threshold (mV) in ‘steady’ trace* | 26.06 (23.14, 28.65) | 24.41 (20.59, 25.50) | NS |
| Action potential width (ms) in ‘steady’ trace* | 1.27 (1.12, 1.32) | 1.39 (1.35, 1.44) | 0.004** |
| Voltage difference between start and end of AP (mV) in ‘steady’ trace* | 15.72 (14.22, 17.20) | 10.52 (9.90, 12.11) | 0.1 × 10−3 *** |
| Amplitude of after‐hyperpolarization (mV) (old method, ‘steady’ trace*) | −0.21 (−0.38, −0.06) | 0.16 (−0.10, 0.76) | 0.01* |
| Duration of after‐hyperpolarization (ms) (old method, ‘steady’ trace*) | 0.23 (0.21, 0.25) | 0.33 (0.27, 4.67) | 0.01* |
| Spike frequency accommodation | 2.76 (1.38, 3.47) | 2.04 (1.11, 3.0) | NS |
| Change in interspike intervals between start and end of step (ms) | 26.15 (11.96, 65.00) | 16.47 (10.63, 30.74) | NS |
| Steepness of interspike interval change vs. AP number relation (ms) | 0.67 (0.21, 2.81) | 0.55 (0.37, 1.19) | NS |
| Change in action potential half‐width during step (ms) | 0.01 (0, 0.03) | 0.02 (−0.06, 0.05) | NS |
| Steepness of AP half‐width change vs. AP number relation (ms) | 0.0004 (−0.0002, 0.0013) | 0.0007 (−0.002, 0.001) | NS |
| Change in action potential amplitude during step (mV) | 2.06 (−2.82, 3.24) | 3.20 (−5.19, 9.65) | NS |
| Steepness of AP amplitude change vs. AP number relation (mV) | 0.04 (−0.07, 0.16) | 0.08 (−0.13, 0.40) | NS |
| Measure of cell excitability | 0.60 (0.44, 1.13) | 0.53 (0.33, 0.81) | NS |
Median values and interquartile ranges for PV+ and CCK+ cells are listed for each physiological feature. The last column shows the P values for comparisons between the medians according to a Mann–Whitney U test where P < 0.05 (NS, non‐significant otherwise); it also marks the level of significance with stars (*P < 0.05; **P < 0.01; ***P < 0.001). The ‘steady’ trace was the response to the smallest current step which evoked at least six action potentials.