A, ABR measurements were conducted in mice from four age groups: Pre‐hearing (P7–9, n = 4–5), Hearing onset (P12–15, n = 7–8), Juvenile (P17–23, n = 21–29) and Young adult (P29–36, n = 5–8). B, ABRs were obtained and average response thresholds (mean ± SEM) were plotted for five frequencies between 8 to 30 kHz and for a click stimulus. Right: averaged ABR waves (± SEM) for each age group are shown in response to 24 kHz tones at 94 dB SPL; waves I–IV are indicated on ABRs. Statistical comparison was performed by a two‐way ANOVA (8–30 kHz frequencies) and one‐way ANOVA (click stimuli). There was a significant decrease in auditory thresholds from Pre‐hearing to Juvenile mice (P < 0.0001) for all frequencies (except at 30 kHz for Pre‐hearing to Hearing onset, P = 0.5035). There was no significant improvement in auditory thresholds from Juvenile to Young adult, except at 12 kHz (P = 0.022).