Figure 2.

Conditional expression of the FLAG-tagged constitutively active IκB kinase β (cIKKβ) transgene results in inflammation during both the canalicular and saccular stage. Doxycycline in drinking water was provided to pregnant dams [embryonic days (E) 13 to 18] or lactating dams [postnatal days (PN) 0 to 2], after which lungs were collected. A–G: FLAG epitope expression in control (CTRL) and inhibitory κB kinase β transactivated (IKTA) lungs at E18 (A–C) and PN2 (D–F) by immunohistochemistry and Western blot analysis. G: Densitometry of FLAG Western blots from E18 and PN2 IKTA lungs. H and I: Gene expression of select cytokines and chemokines in E18 (H) and PN2 (I) lungs measured by quantitative RT-PCR. Data are expressed as means ± SEM. n = 4 per group (G); n = 6 pups per group (H and I). ^∗P < 0.05. Original magnification, ×400.