Figure 4.

Ileocecal resection (ICR) decreases Trp53 nuclear accumulation and activity. Western blot (WB) analysis and densitometry of intestinal epithelial cell (IEC) nuclear fractions from control (ctl), sham, and ICR mice for total Trp53 (A), p-Trp53^S9 (B), and acetyl-Trp53^K370 (C). TATA binding protein (TBP) was used as a loading control (as in Figure 2E). D: Real-time RT-PCR for the Trp53 targets Dr5 and puma indicate decreased Trp53 activity in ICR compared with control or sham mice. E: WB analysis of IEC cytosolic fractions from control, sham, and ICR mice for (Pik3r1) p85α. Data are shown as the means ± SEM (A–E). n = 3 (A–C and E, ctl, sham, and ICR; D, ctl and sham); n = 6 (D, ICR). ^∗P < 0.05 versus control.