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. 2016 Jul;186(7):1837–1846. doi: 10.1016/j.ajpath.2016.03.008

Table 2.

Quantitative RT-PCR Primer Sequences

Gene Sequence
Gapdh F 5′-GAACGGATTTGGCCGTATTG-3′
R 5′-TGAGTGGAGTCATACTGGAACATGT-3′
Lgr5 F 5′-CAAACTTCCCAGAGCTCAAGATTAT-3′
R 5′-TGTTGCCGTCGTCTTTATTCC-3′
c-Myc F 5′-CCACCAGCAGCGACTCTGA-3′
R 5′-TCCACAGACACCACATCAATTTC-3′
Cd44 F 5′-AGCAGCGGCTCCACCATCGAGA-3′
R 5′-TCGGATCCATGAGTCACAGTG-3′
Axin2 F 5′-CGCCAACGACAGCGAGTTAT-3′
R 5′-CCATCTACGCTACTGTCCGTCAT-3′
Birc5 (survivin) F 5′-ATCGCCACCTTCAAGAACTG-3′
R 5′-GGCCAAATCAGGCTCGTTCT-3′
Dr5 F 5′-CGGGCAGATCACTACACCC-3′
R 5′-TGTTACTGGAACAAAGACAGCC-3′
Bbc3 (puma) F 5′-ATGGCGGACGACCTCAAC-3′
R 5′-AGTCCCATGAAGAGATTGTACATGAC-3′

F, forward; R, reverse.