Supplemental Figure S2.

Immunohistochemistry of AML cells in vivo correlates with LEC differentiation found in TSC2⁻ cells. In AML, cells have a relatively weak nuclear immunoreactivity for PROX1 compared with normal LECs (arrows) and are negative for podoplanin, which is strongly positive in normal LECs (arrow). Notice the weak cytoplasmic PROX1 immunoreaction. Hematoxylin and eosin reveals the typical histologic features of AML. AML, angiomyolipoma; LEC, lymphatic endothelial cell; TSC2, tuberous sclerosis complex-2.