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. 2016 Jul;186(7):1825–1836. doi: 10.1016/j.ajpath.2016.03.009

Figure 2.

Figure 2

Expression of NCC markers in AML cells with or without TSC2 correction does not support an NCC lineage derivation. Western blot showing absence of NCC precursor markers nestin and P75R, absence of NCC markers TWIST and PHOX2b in TSC2− and TSC2+ cells, disappearance of NCC marker SOX10, and decreased expression of NCC markers SOX10, SNAIL2, FOXD3, and SOX9 in TSC2+ cells compared with TSC2− cells. GAPDH detection indicates equal loading. Histograms show density of Western blot signals in TSC2+ cells relative to TSC2− cells, as analyzed by ImageJ software version 1.47 (NIH, Bethesda, MD; http://imagej.nih.gov/ij). AML, angiomyolipoma; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NCC, neural crest cell; TSC2, tuberous sclerosis complex-2.