Figure 3.

Recombinant Delta-like ligand 4 (rDll4) decreases carbon tetrachloride (CCl₄) -induced inflammatory cell recruitment and cytokine/chemokine expression. A: Hematoxylin and eosin staining was performed to monitor inflammation in CCl₄-induced liver damage. Boxed area is highly magnified on the left to highlight inflammatory cell infiltration in CCl₄-challenged mice. F4/80 and CD45 staining were performed to visualize inflammatory cells. B: Semiquantification for F4/80 and CD45 staining was performed using Leica microscope imaging software. mRNA expression of inflammatory cytokines (C) and chemokines (D) was measured by real-time quantitative PCR (qPCR). qPCR measurements were repeated at least three times. Data are presented as means ± SEM (B and C). n = 6 in each group (A). ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗∗P < 0.0001. Scale bars: 100 μm (A, top panels); 50 μm (A, middle and bottom panels); 20 μm (A, magnification).