Figure 6.

Recombinant Delta-like ligand 4 (rDll4) rapidly causes massive hepatic necrosis in bile duct ligation (BDL) mice. A: The diameter of bile infarcts at different time points was calculated in BDL-administered mice with or without rDll4 injection. For each liver, diameters were measured in three areas with maximum bile infarct. B: Liver tissues from representative mice (BDL versus BDL + rDll4) showing bile infarct at 24 hours and at day 7 after BDL. Real-time quantitative PCR (C) and immunohistochemical (D) analyses reveal that rDll4 administration reduces expression of chemokine ligand (Ccl)2 in mice after 4 days of BDL. Data are presented as means ± SEM (A and C). n = 6 in each group (A). ^∗P < 0.05, ^∗∗∗∗P < 0.0001. Scale bars = 100 μm (B and D).