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. 2016 Jun 16;7(6):27. doi: 10.3390/genes7060027

Table 2.

Primers used in qPCR. * Two annealing temperatures are provided for the hTERT reverse primer since the temperature was dependent on the respective forward primer used (WT or α hTERT).

Primer Sequence Annealing Temperature (°C) Ref.
hGAPDH fw TGCACCACCAACTGCTTAGC 60 [37]
hGAPDH rev GGCATGGACTGTGGTCATGA 60 [37]
hTR fw GCCTTCCACCGTTCATTCTA 60 [38]
hTR rev CCTGAAAGGCCTGAACCTC 60 [38]
WT hTERT fw TGTACTTTGTCAAGGTGGATGTG 60 [14]
α-hTERT fw CTGAGCTGTACTTTGTCAAGGAC 53 [15]
hTERT rev GTACGGCTGGAGGTCTGTCAA 60/53 * [14,15]