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. 2016 Jul 1;6:28968. doi: 10.1038/srep28968

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) miRNA microarray analysis illustrates expression profiles of miRNAs that are differentially expressed upon HPV16 E6 overexpression in HEK293T cells. Green, decreased expression; Red, increased expression. (B) Disease and function analysis (Ingenuity IPA software) of the above 59 differential changes shows the 20 main influenced diseases and functions in HEK293T cells expressing HPV16 E6. (C) Verification of selectively 15 altered expression of miRNAs in HEK293T cells following overexpression of HPV16 E6 by qRT-PCR analysis. (D) Verification of the altered miRNAs in p53-mutant HaCaT cells expressing HPV16 E6 or empty vector. Error bars represent ± SD of three experiments. *P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant.