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. 2016 Jul 1;6:28968. doi: 10.1038/srep28968

Figure 2. miR-2861 is regulated by HPV16 E6 and exerts tumor suppressive roles in cervical cancer SiHa and CaSki cells.

Figure 2

(A) HPV16 E6 negatively regulates miR-2861 expression in both SiHa and CaSki cells. SiHa and CaSki cell lines were transfected with specific siRNA targeting HPV16 E6 or scrambled siRNA (left 2 lanes), and plasmid expression HPV16 E6 or empty vector (right 2 lanes) for 48 h, respectively. miR-2861 expression was assessed by qRT-PCR. (B) miR-2861 inhibits cell proliferation of SiHa and CaSki cells. Cells were plated in each well and transfected with miR-2861 or miR-NC. CCK8 assay was then performed at the time indicated. (C) miR-2861 enhances apoptosis of SiHa and CaSki cells. At 48 h after transfection with miR-2861 or miR-NC, SiHa and CaSki cells were collected for apoptosis assay using flow cytometry, respectively. **P < 0.01; ***P < 0.001.