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. 2016 Jul 1;6:28968. doi: 10.1038/srep28968

Figure 3. miR-2861 inhibits the migration and invasion of cervical cancer cells and is negatively associated with advanced tumor stage and lymph node metastasis.

Figure 3

(A,C) miR-2861 inhibits the migration and invasion of SiHa (A) and CaSki (C) cells. At 24 h of posttransfection, cells were collected for migration (top) and matrigel invasion assays (bottom). Representative images are shown (magnification: ×200). The results are plotted as the average number of migrated or invasive cells from 5 randomly selected fields. Data are represented as the mean ± SD of three independent experiments. (B,D) miR-2861 increased E-cadherin expression level in SiHa (B) and CaSki (D) cells. 72 h after transfection, the protein level of E-cadherin was analyzed by Western blot. (E) miR-2861 is reduced in HPV16 positive cervical cancer tissues. qRT-PCR was performed to examine miR-2861 expression in 20 normal cervix and 57 HPV16 positive cervical cancer tissues. (F) miR-2861 is decreased in cervical cancer tissues with advanced tumor stage. qRT-PCR was used to assess miR-2861 expression. (G) miR-2861 is decreased in cervical cancer tissues with lymph node metastasis. qRT-PCR was used to analyze miR-2861 expression. *P < 0.05; **P < 0.01; ***P < 0.001.