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. 2016 Jul 1;6:28968. doi: 10.1038/srep28968

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(A,B) A matrigel invasion assay was performed in SiHa (A) and CaSki (B) cells transfected with si-EGFR, si-AKT2, si-CCND1, or si-NS. Representative images are shown (magnification: ×200). Error bars indicate ± SD. (C) Suppression of EGFR, AKT2, or CCND1 increased E-cadherin expression level in both SiHa and CaSki cells. 72 h after transfection, the protein level of E-cadherin was analyzed by Western blot. **P < 0.01; ***P < 0.001.