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. 2016 Jul 1;6:28894. doi: 10.1038/srep28894

Figure 1. Non-enzymatic DNA cleavage at an AP site.

Figure 1

AP site-containing DNA was obtained by treating the corresponding dU-containing DNA with UDG. (a) Structure of a representative catalyst for DNA scission. (b) DNA substrates. (c) Scission of 8-oxo-dG-containing DNA (250 nM) in the presence of hOGG1 (100 nM) and C1 (10 μM). (d) Scission of AP site-containing DNA (250 nM) in the presence of hOGG1 (50 nM) and C1 (10 μM). (e) Scission of AP site-containing DNA (250 nM) in the presence of hNEIL1 (50 nM) and C1 (10 μM). (f) Scission of AP site-containing DNA (2 μM) by C1. The percent of products was corrected for the spontaneous cleavage. Reactions were carried out at 37 °C for 30 min (b–d) or 16 h (e).