Figure 1. Humoral immune responses in mice vaccinated with AV-1959R protein formulated with cGMP grade adjuvants (Advax^CpG, Advax^TM, Montanide-ISA51, Montanide-ISA720, MPLA, Alhydrogel®) and control adjuvant, Quil-A.

(a) Concentrations of anti-Aβ antibodies were measured by ELISA in sera collected after the 3^rd immunization. Lines represent mean values. (b) Isotypes of generated anti-Aβ antibodies had been determined by ELISA (see Fig. S1) and IgG1/IgG2a^b ratio was calculated. Bars represent average ± SD (n = 6–8/per group). Statistical significance was calculated against group of mice immunized with AV-1959R formulated in Advax^CpG using ANOVA test (**P < 0.01***P < 0.001 and ****P < 0.0001).