Figure 2. Cellular immune responses in mice vaccinated with AV-1959R protein formulated with cGMP grade adjuvants (Advax^CpG, Advax^TM, Montanide-ISA51, Montanide-ISA720, MPLA, Alhydrogel®) and control adjuvant, Quil-A.

(a,b) Numbers of IFN-γ (a) and IL-4 (b) producing T cells were calculated by ELISpot in splenocyte cultures obtained from experimental and control animals. (c) IL-4/IFN-γ ratios were calculated based on data presented in (a,b). Bars represent average ± SD (n = 6–8/per group). Statistical significances were calculated against group of mice immunized with AV-1959R formulated in Advax^CpG using ANOVA test (*P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001).