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. 2016 Jul 1;6:28811. doi: 10.1038/srep28811

Figure 1. TPβ is highly expressed in IUGR placentae but is absent from healthy placentae.

Figure 1

(a) Slot blots on lysates from normal, healthy placentae and placentae from pregnancies complicated by IUGR were probed with antibodies to identify total TP expression and that of the two isoforms (TPα and TPβ) in addition to a loading control (Histone H1) (n = 17 control and 15 IUGR placentae). (b) IHC on paraffin-embedded sections from normal, healthy placentae and placentae from pregnancies complicated by IUGR were probed with antibodies to TPα and TPβ. Sections were imaged at 200x magnification, scale bar: 100 μm; arrows indicate staining in vascular endothelium (n = 53 control and 22 IUGR placentae). (c) Quantitative gene expression of total TP, TPα and TPβ (and change in the ratio of TPα:TPβ) relative to HPRT1 expression was assessed in the same placental samples analysed in (a) (n = 13 control and 12 IUGR placentae). Data represent individual samples (mean ± SEM); Independent samples T-test, NS: not significant, *P < 0.05, **P < 0.01.