Figure 2. Differential effects of TP isoforms on trophoblast cell cycle.

(a) Proliferation of BeWo cells (□) transfected with empty vector-∅ (), TPΔ³²⁸ (O), TPα (■) and TPβ (●) was monitored over 168 hours. (b) Proliferation of JEG-3 cells expressing similar constructs, stimulated with vehicle (□) or 100 nM I-BOP (■) was documented over the same period. (c) Apoptosis of BeWo cells under basal (□) and serum-deprivation (■) was assessed after 24 hours using flow cytometry. (d) Distribution of transfected BeWo lines in phases of the cell cycle (G0/G1 □, S , G2/M ■) was assessed also using flow cytometry. The data are represented as mean ± SEM and are representative of 3 independent experiments. One-way ANOVA and Independent samples T-test, *P < 0.05, **P < 0.01, ***P < 0.005.