Figure 3. TP isoforms differentially regulate the molecular machinery controlling cell cycle progression.

Immunoblotting of BeWo cells transfected with empty vector (∅), TPΔ³²⁸, TPα and TPβ was used to examine pRb phosphorylation (a), Cyclins (b), Cyclin-dependent kinases (CDK) (c) and tumour suppressor proteins (d). Non-phosphorylated Rb was used as the loading control in panel (a) and GAPDH was used as the loading control in panels (b-d). Blots are representative of 4 individual experiments. Densitometry was used to quantify changes in protein expression and activation status.