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. 2015 Oct 12;35(25):3324–3334. doi: 10.1038/onc.2015.394

Figure 3.

Figure 3

KLF12 promotes anoikis. (a) KLF12 knockdown results in decreased viability in suspension. A549, H23, H460 and HOP62 PAR cells infected with shRNA against KLF12 or scramble control were grown in plastic or polyHEMA and their viability was assessed (upper panels). The statistical significance was determined by Hochberg analysis (n=3) and the P-values were 0.02, 0.02, 0.02 and 0.008 for A549, H23, H460 and HOP62, respectively. KLF12 knockdown efficiently was determined in triplicate by Q-PCR (lower panels), and the statistical significance was determined by Hochberg analysis. The P-values were 0.0005, 0.005, 0.005 and 0.0004 for A549, H23, H460 and HOP62, respectively. (b) KLF12 knockdown leads to decreased apoptosis in suspension. A549 PAR cells infected with shRNA against KLF12 or scramble control were grown in polyHEMA or plastic and the Annexin-positive apoptotic population was quantified by fluorescence-activated cell sorting analysis (left). Histogram showing the Annexin-positive population as an average of four independent experiments (right). Following a two-way analysis of variance, the P-values were determined to be 0.00008 looking at the KLF12 effect within polyHEMA and 0.0005 looking at the polyHEMA effect within shSCR (n=4).