A, Peritoneal cell counts in lavages collected 0, 4, 24, and 96 hours after the injection of 300 μL of 10% thioglycollate. Flow cytometry was used to determine the number of 11β-HSD1+ peritoneal cells (as a percentage of total cells) (B) and the MFI of cellular 11β-HSD1 expression (C) during the course of peritonitis. D, Real-time PCR measurement of Hsd11b1 mRNA levels (relative to Hprt) in total peritoneal cells 4, 24, and 96 hours after the injection of 300 μL of 10% thioglycollate, expressed in arbitrary units (AU), with levels at 4 hours arbitrarily set to 1.0. Data are means ± SEM and were analyzed by an ANOVA, with Tukey's post hoc tests. *, P < .05, **, P < .01, ***, P < .001, compared with 0 hours; §, P < .001 compared with 4 hours; ¶, P < .001 compared with 24 hours (n = 6–8/group).