Skip to main content
. 2016 May 4;157(7):2928–2936. doi: 10.1210/en.2016-1118

Figure 6. 11β-HSD1 inhibition enhances surface expression of adhesion molecule CD11b on peritoneal neutrophils.

Figure 6.

Flow cytometry was used to measure surface expression of CD11b on peritoneal cells lavaged 4 hours after the thioglycollate injection. A, CD11b MFI was higher on the peritoneal neutrophils (7/4+Ly6G+) from mice pretreated with UE2316 (white bar) than from mice pretreated with vehicle (black bar). B, No difference was found in surface CD11b MFI on monocytes (7/4+Ly6G−) between the two groups. Data are means ± SEM and were analyzed by a Students t test. *, P < .05 (n = 9–10). Levels of mRNA encoding CD11b (C) and L-selectin (D) were measured relative to mRNA encoding HPRT in peritoneal cells lavaged 4 hours after the thioglycollate injection from mice pretreated with vehicle (Veh, black bars) or UE2316 (white bars), with levels in Veh-treated mice arbitrarily set to 1.0. Data are means ± SEM and were analyzed by a Student's t test. *, P < .05 (n = 8–9). HPRT, hypoxanthine-guanine phosphoribosyl transferase.