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. 2016 Jul 1;6:29045. doi: 10.1038/srep29045

Figure 2. The morphology produced by vph1 mutations is atypical among mutants lacking V-ATPase pump activity.

Figure 2

BY4741 cells were grown in buffered media to logarithmic phase, stained with FM4-64, and visualized by fluorescence microscopy and DIC. Scale bar: 5 μm. (A) Cells with deletions in various V1 subunits. (B) vph1Δ cells reconstituted with plasmids expressing the indicated alleles. (C) Cells from panel B were loaded with BCECF-AM and vacuolar pH was determined by fluorescence spectroscopy. Error bars represent Standard Deviation. (n = 9–22) (D) Total cell extracts of the cells from panel B were analyzed by SDS-PAGE and Western blotting against Vph1 and actin (Act1).